Cloning, expression, purification, and characterization of glutaredoxin from Antarctic sea-ice bacterium Pseudoalteromonas sp. AN178.
Identifieur interne : 000671 ( Main/Exploration ); précédent : 000670; suivant : 000672Cloning, expression, purification, and characterization of glutaredoxin from Antarctic sea-ice bacterium Pseudoalteromonas sp. AN178.
Auteurs : Quanfu Wang [République populaire de Chine] ; Yanhua Hou [République populaire de Chine] ; Yonglei Shi [République populaire de Chine] ; Xiao Han [République populaire de Chine] ; Qian Chen [République populaire de Chine] ; Zhiguo Hu [République populaire de Chine] ; Yuanping Liu [République populaire de Chine] ; Yujin Li [République populaire de Chine]Source :
- BioMed research international [ 2314-6141 ] ; 2014.
Descripteurs français
- KwdFr :
- Alignement de séquences (MeSH), Analyse de séquence d'ADN (MeSH), Clonage moléculaire (MeSH), Données de séquences moléculaires (MeSH), Glace (MeSH), Glutarédoxines (composition chimique), Glutarédoxines (génétique), Glutarédoxines (isolement et purification), Protéines recombinantes (composition chimique), Protéines recombinantes (isolement et purification), Pseudoalteromonas (génétique), Pseudoalteromonas (métabolisme), Régions antarctiques (MeSH), Séquence d'acides aminés (MeSH), Électrophorèse sur gel de polyacrylamide (MeSH).
- MESH :
- composition chimique : Glutarédoxines, Protéines recombinantes.
- génétique : Glutarédoxines, Pseudoalteromonas.
- isolement et purification : Glutarédoxines, Protéines recombinantes.
- métabolisme : Pseudoalteromonas.
- Alignement de séquences, Analyse de séquence d'ADN, Clonage moléculaire, Données de séquences moléculaires, Glace, Régions antarctiques, Séquence d'acides aminés, Électrophorèse sur gel de polyacrylamide.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Antarctic Regions (MeSH), Cloning, Molecular (MeSH), Electrophoresis, Polyacrylamide Gel (MeSH), Glutaredoxins (chemistry), Glutaredoxins (genetics), Glutaredoxins (isolation & purification), Ice (MeSH), Molecular Sequence Data (MeSH), Pseudoalteromonas (genetics), Pseudoalteromonas (metabolism), Recombinant Proteins (chemistry), Recombinant Proteins (isolation & purification), Sequence Alignment (MeSH), Sequence Analysis, DNA (MeSH).
- MESH :
- chemical , chemistry : Glutaredoxins, Recombinant Proteins.
- chemical , genetics : Glutaredoxins.
- chemical , isolation & purification : Glutaredoxins, Recombinant Proteins.
- geographic : Antarctic Regions, Ice.
- genetics : Pseudoalteromonas.
- metabolism : Pseudoalteromonas.
- Amino Acid Sequence, Cloning, Molecular, Electrophoresis, Polyacrylamide Gel, Molecular Sequence Data, Sequence Alignment, Sequence Analysis, DNA.
Abstract
Glutaredoxins (Grxs) are small ubiquitous redox enzymes that catalyze glutathione-dependent reactions to reduce protein disulfide. In this study, a full-length Grx gene (PsGrx) with 270 nucleotides was isolated from Antarctic sea-ice bacterium Pseudoalteromonas sp. AN178. It encoded deduced 89 amino acid residues with the molecular weight 9.8 kDa. Sequence analysis of the amino acid sequence revealed the catalytic motif CPYC. Recombinant PsGrx (rPsGrx) stably expressed in E. coli BL21 was purified to apparent homogeneity by Ni-affinity chromatography. rPsGrx exhibited optimal activity at 30°C and pH 8.0 and showed 25.5% of the activity at 0°C. It retained 65.0% of activity after incubation at 40°C for 20 min and still exhibited 37.0% activity in 1.0 M NaCl. These results indicated that rPsGrx was a typical cold active protein with low thermostability.
DOI: 10.1155/2014/246871
PubMed: 25110664
PubMed Central: PMC4109671
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Cloning, expression, purification, and characterization of glutaredoxin from Antarctic sea-ice bacterium Pseudoalteromonas sp. AN178.</title><author><name sortKey="Wang, Quanfu" sort="Wang, Quanfu" uniqKey="Wang Q" first="Quanfu" last="Wang">Quanfu Wang</name><affiliation wicri:level="1"><nlm:affiliation>School of Marine and Technology, Harbin Institute of Technology, Weihai 264209, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Marine and Technology, Harbin Institute of Technology, Weihai 264209</wicri:regionArea><wicri:noRegion>Weihai 264209</wicri:noRegion></affiliation></author><author><name sortKey="Hou, Yanhua" sort="Hou, Yanhua" uniqKey="Hou Y" first="Yanhua" last="Hou">Yanhua Hou</name><affiliation wicri:level="1"><nlm:affiliation>School of Marine and Technology, Harbin Institute of Technology, Weihai 264209, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Marine and Technology, Harbin Institute of Technology, Weihai 264209</wicri:regionArea><wicri:noRegion>Weihai 264209</wicri:noRegion></affiliation></author><author><name sortKey="Shi, Yonglei" sort="Shi, Yonglei" uniqKey="Shi Y" first="Yonglei" last="Shi">Yonglei Shi</name><affiliation wicri:level="1"><nlm:affiliation>School of Marine and Technology, Harbin Institute of Technology, Weihai 264209, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Marine and Technology, Harbin Institute of Technology, Weihai 264209</wicri:regionArea><wicri:noRegion>Weihai 264209</wicri:noRegion></affiliation></author><author><name sortKey="Han, Xiao" sort="Han, Xiao" uniqKey="Han X" first="Xiao" last="Han">Xiao Han</name><affiliation wicri:level="1"><nlm:affiliation>School of Marine and Technology, Harbin Institute of Technology, Weihai 264209, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Marine and Technology, Harbin Institute of Technology, Weihai 264209</wicri:regionArea><wicri:noRegion>Weihai 264209</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Qian" sort="Chen, Qian" uniqKey="Chen Q" first="Qian" last="Chen">Qian Chen</name><affiliation wicri:level="1"><nlm:affiliation>School of Marine and Technology, Harbin Institute of Technology, Weihai 264209, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Marine and Technology, Harbin Institute of Technology, Weihai 264209</wicri:regionArea><wicri:noRegion>Weihai 264209</wicri:noRegion></affiliation></author><author><name sortKey="Hu, Zhiguo" sort="Hu, Zhiguo" uniqKey="Hu Z" first="Zhiguo" last="Hu">Zhiguo Hu</name><affiliation wicri:level="1"><nlm:affiliation>School of Marine and Technology, Harbin Institute of Technology, Weihai 264209, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Marine and Technology, Harbin Institute of Technology, Weihai 264209</wicri:regionArea><wicri:noRegion>Weihai 264209</wicri:noRegion></affiliation></author><author><name sortKey="Liu, Yuanping" sort="Liu, Yuanping" uniqKey="Liu Y" first="Yuanping" last="Liu">Yuanping Liu</name><affiliation wicri:level="1"><nlm:affiliation>Shandong Provincial Engineering Technology Research Center of Marine Health Food (Taixiang Group), Rongcheng 264300, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shandong Provincial Engineering Technology Research Center of Marine Health Food (Taixiang Group), Rongcheng 264300</wicri:regionArea><wicri:noRegion>Rongcheng 264300</wicri:noRegion></affiliation></author><author><name sortKey="Li, Yujin" sort="Li, Yujin" uniqKey="Li Y" first="Yujin" last="Li">Yujin Li</name><affiliation wicri:level="1"><nlm:affiliation>Shandong Provincial Key Laboratory of Processing Technology of Frozen Prepared Food (Taixiang Group), Rongcheng 264300, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shandong Provincial Key Laboratory of Processing Technology of Frozen Prepared Food (Taixiang Group), Rongcheng 264300</wicri:regionArea><wicri:noRegion>Rongcheng 264300</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014</date><idno type="RBID">pubmed:25110664</idno><idno type="pmid">25110664</idno><idno type="doi">10.1155/2014/246871</idno><idno type="pmc">PMC4109671</idno><idno type="wicri:Area/Main/Corpus">000603</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000603</idno><idno type="wicri:Area/Main/Curation">000603</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000603</idno><idno type="wicri:Area/Main/Exploration">000603</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Cloning, expression, purification, and characterization of glutaredoxin from Antarctic sea-ice bacterium Pseudoalteromonas sp. AN178.</title><author><name sortKey="Wang, Quanfu" sort="Wang, Quanfu" uniqKey="Wang Q" first="Quanfu" last="Wang">Quanfu Wang</name><affiliation wicri:level="1"><nlm:affiliation>School of Marine and Technology, Harbin Institute of Technology, Weihai 264209, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Marine and Technology, Harbin Institute of Technology, Weihai 264209</wicri:regionArea><wicri:noRegion>Weihai 264209</wicri:noRegion></affiliation></author><author><name sortKey="Hou, Yanhua" sort="Hou, Yanhua" uniqKey="Hou Y" first="Yanhua" last="Hou">Yanhua Hou</name><affiliation wicri:level="1"><nlm:affiliation>School of Marine and Technology, Harbin Institute of Technology, Weihai 264209, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Marine and Technology, Harbin Institute of Technology, Weihai 264209</wicri:regionArea><wicri:noRegion>Weihai 264209</wicri:noRegion></affiliation></author><author><name sortKey="Shi, Yonglei" sort="Shi, Yonglei" uniqKey="Shi Y" first="Yonglei" last="Shi">Yonglei Shi</name><affiliation wicri:level="1"><nlm:affiliation>School of Marine and Technology, Harbin Institute of Technology, Weihai 264209, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Marine and Technology, Harbin Institute of Technology, Weihai 264209</wicri:regionArea><wicri:noRegion>Weihai 264209</wicri:noRegion></affiliation></author><author><name sortKey="Han, Xiao" sort="Han, Xiao" uniqKey="Han X" first="Xiao" last="Han">Xiao Han</name><affiliation wicri:level="1"><nlm:affiliation>School of Marine and Technology, Harbin Institute of Technology, Weihai 264209, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Marine and Technology, Harbin Institute of Technology, Weihai 264209</wicri:regionArea><wicri:noRegion>Weihai 264209</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Qian" sort="Chen, Qian" uniqKey="Chen Q" first="Qian" last="Chen">Qian Chen</name><affiliation wicri:level="1"><nlm:affiliation>School of Marine and Technology, Harbin Institute of Technology, Weihai 264209, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Marine and Technology, Harbin Institute of Technology, Weihai 264209</wicri:regionArea><wicri:noRegion>Weihai 264209</wicri:noRegion></affiliation></author><author><name sortKey="Hu, Zhiguo" sort="Hu, Zhiguo" uniqKey="Hu Z" first="Zhiguo" last="Hu">Zhiguo Hu</name><affiliation wicri:level="1"><nlm:affiliation>School of Marine and Technology, Harbin Institute of Technology, Weihai 264209, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Marine and Technology, Harbin Institute of Technology, Weihai 264209</wicri:regionArea><wicri:noRegion>Weihai 264209</wicri:noRegion></affiliation></author><author><name sortKey="Liu, Yuanping" sort="Liu, Yuanping" uniqKey="Liu Y" first="Yuanping" last="Liu">Yuanping Liu</name><affiliation wicri:level="1"><nlm:affiliation>Shandong Provincial Engineering Technology Research Center of Marine Health Food (Taixiang Group), Rongcheng 264300, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shandong Provincial Engineering Technology Research Center of Marine Health Food (Taixiang Group), Rongcheng 264300</wicri:regionArea><wicri:noRegion>Rongcheng 264300</wicri:noRegion></affiliation></author><author><name sortKey="Li, Yujin" sort="Li, Yujin" uniqKey="Li Y" first="Yujin" last="Li">Yujin Li</name><affiliation wicri:level="1"><nlm:affiliation>Shandong Provincial Key Laboratory of Processing Technology of Frozen Prepared Food (Taixiang Group), Rongcheng 264300, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shandong Provincial Key Laboratory of Processing Technology of Frozen Prepared Food (Taixiang Group), Rongcheng 264300</wicri:regionArea><wicri:noRegion>Rongcheng 264300</wicri:noRegion></affiliation></author></analytic><series><title level="j">BioMed research international</title><idno type="eISSN">2314-6141</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Antarctic Regions (MeSH)</term><term>Cloning, Molecular (MeSH)</term><term>Electrophoresis, Polyacrylamide Gel (MeSH)</term><term>Glutaredoxins (chemistry)</term><term>Glutaredoxins (genetics)</term><term>Glutaredoxins (isolation & purification)</term><term>Ice (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Pseudoalteromonas (genetics)</term><term>Pseudoalteromonas (metabolism)</term><term>Recombinant Proteins (chemistry)</term><term>Recombinant Proteins (isolation & purification)</term><term>Sequence Alignment (MeSH)</term><term>Sequence Analysis, DNA (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alignement de séquences (MeSH)</term><term>Analyse de séquence d'ADN (MeSH)</term><term>Clonage moléculaire (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Glace (MeSH)</term><term>Glutarédoxines (composition chimique)</term><term>Glutarédoxines (génétique)</term><term>Glutarédoxines (isolement et purification)</term><term>Protéines recombinantes (composition chimique)</term><term>Protéines recombinantes (isolement et purification)</term><term>Pseudoalteromonas (génétique)</term><term>Pseudoalteromonas (métabolisme)</term><term>Régions antarctiques (MeSH)</term><term>Séquence d'acides aminés (MeSH)</term><term>Électrophorèse sur gel de polyacrylamide (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Glutaredoxins</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Glutaredoxins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Glutaredoxins</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Antarctic Regions</term><term>Ice</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Glutarédoxines</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Pseudoalteromonas</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glutarédoxines</term><term>Pseudoalteromonas</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Glutarédoxines</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Pseudoalteromonas</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Pseudoalteromonas</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Cloning, Molecular</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Molecular Sequence Data</term><term>Sequence Alignment</term><term>Sequence Analysis, DNA</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Alignement de séquences</term><term>Analyse de séquence d'ADN</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Glace</term><term>Régions antarctiques</term><term>Séquence d'acides aminés</term><term>Électrophorèse sur gel de polyacrylamide</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Glutaredoxins (Grxs) are small ubiquitous redox enzymes that catalyze glutathione-dependent reactions to reduce protein disulfide. In this study, a full-length Grx gene (PsGrx) with 270 nucleotides was isolated from Antarctic sea-ice bacterium Pseudoalteromonas sp. AN178. It encoded deduced 89 amino acid residues with the molecular weight 9.8 kDa. Sequence analysis of the amino acid sequence revealed the catalytic motif CPYC. Recombinant PsGrx (rPsGrx) stably expressed in E. coli BL21 was purified to apparent homogeneity by Ni-affinity chromatography. rPsGrx exhibited optimal activity at 30°C and pH 8.0 and showed 25.5% of the activity at 0°C. It retained 65.0% of activity after incubation at 40°C for 20 min and still exhibited 37.0% activity in 1.0 M NaCl. These results indicated that rPsGrx was a typical cold active protein with low thermostability. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25110664</PMID><DateCompleted><Year>2015</Year><Month>03</Month><Day>31</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">2314-6141</ISSN><JournalIssue CitedMedium="Internet"><Volume>2014</Volume><PubDate><Year>2014</Year></PubDate></JournalIssue><Title>BioMed research international</Title><ISOAbbreviation>Biomed Res Int</ISOAbbreviation></Journal><ArticleTitle>Cloning, expression, purification, and characterization of glutaredoxin from Antarctic sea-ice bacterium Pseudoalteromonas sp. AN178.</ArticleTitle><Pagination><MedlinePgn>246871</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1155/2014/246871</ELocationID><Abstract><AbstractText>Glutaredoxins (Grxs) are small ubiquitous redox enzymes that catalyze glutathione-dependent reactions to reduce protein disulfide. In this study, a full-length Grx gene (PsGrx) with 270 nucleotides was isolated from Antarctic sea-ice bacterium Pseudoalteromonas sp. AN178. It encoded deduced 89 amino acid residues with the molecular weight 9.8 kDa. Sequence analysis of the amino acid sequence revealed the catalytic motif CPYC. Recombinant PsGrx (rPsGrx) stably expressed in E. coli BL21 was purified to apparent homogeneity by Ni-affinity chromatography. rPsGrx exhibited optimal activity at 30°C and pH 8.0 and showed 25.5% of the activity at 0°C. It retained 65.0% of activity after incubation at 40°C for 20 min and still exhibited 37.0% activity in 1.0 M NaCl. These results indicated that rPsGrx was a typical cold active protein with low thermostability. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Quanfu</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>School of Marine and Technology, Harbin Institute of Technology, Weihai 264209, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hou</LastName><ForeName>Yanhua</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>School of Marine and Technology, Harbin Institute of Technology, Weihai 264209, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shi</LastName><ForeName>Yonglei</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>School of Marine and Technology, Harbin Institute of Technology, Weihai 264209, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Han</LastName><ForeName>Xiao</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>School of Marine and Technology, Harbin Institute of Technology, Weihai 264209, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Qian</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>School of Marine and Technology, Harbin Institute of Technology, Weihai 264209, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hu</LastName><ForeName>Zhiguo</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>School of Marine and Technology, Harbin Institute of Technology, Weihai 264209, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Yuanping</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Shandong Provincial Engineering Technology Research Center of Marine Health Food (Taixiang Group), Rongcheng 264300, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>YuJin</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Shandong Provincial Key Laboratory of Processing Technology of Frozen Prepared Food (Taixiang Group), Rongcheng 264300, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName><AccessionNumberList><AccessionNumber>KF361316</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>07</Month><Day>07</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Biomed Res Int</MedlineTA><NlmUniqueID>101600173</NlmUniqueID></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D054477">Glutaredoxins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007053">Ice</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000864" MajorTopicYN="N" Type="Geographic">Antarctic Regions</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004591" MajorTopicYN="N">Electrophoresis, Polyacrylamide Gel</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D054477" MajorTopicYN="N">Glutaredoxins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007053" MajorTopicYN="Y">Ice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D031161" MajorTopicYN="N">Pseudoalteromonas</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016415" MajorTopicYN="N">Sequence Alignment</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year><Month>04</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2014</Year><Month>06</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2014</Year><Month>06</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>8</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>8</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>4</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25110664</ArticleId><ArticleId IdType="doi">10.1155/2014/246871</ArticleId><ArticleId IdType="pmc">PMC4109671</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>J Agric Food Chem. 2009 Nov 11;57(21):10357-62</Citation><ArticleIdList><ArticleId IdType="pubmed">19886686</ArticleId></ArticleIdList></Reference><Reference><Citation>Microbiol Res. 2014 Feb-Mar;169(2-3):179-84</Citation><ArticleIdList><ArticleId IdType="pubmed">23890723</ArticleId></ArticleIdList></Reference><Reference><Citation>J Bacteriol. 2010 Mar;192(5):1370-6</Citation><ArticleIdList><ArticleId IdType="pubmed">20061476</ArticleId></ArticleIdList></Reference><Reference><Citation>Antioxid Redox Signal. 2010 Mar 15;12(6):787-92</Citation><ArticleIdList><ArticleId IdType="pubmed">19769456</ArticleId></ArticleIdList></Reference><Reference><Citation>J Mol Biol. 2010 May 14;398(4):614-22</Citation><ArticleIdList><ArticleId IdType="pubmed">20347849</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Microbiol. 2011 Feb;62(2):544-6</Citation><ArticleIdList><ArticleId IdType="pubmed">20730433</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Cell Biol. 1999 Dec;19(12):8180-90</Citation><ArticleIdList><ArticleId IdType="pubmed">10567543</ArticleId></ArticleIdList></Reference><Reference><Citation>J Mol Biol. 2001 Jul 20;310(4):907-18</Citation><ArticleIdList><ArticleId IdType="pubmed">11453697</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2001 Aug 10;276(32):30374-80</Citation><ArticleIdList><ArticleId IdType="pubmed">11397793</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 1989 Aug 25;264(24):13963-6</Citation><ArticleIdList><ArticleId IdType="pubmed">2668278</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods Enzymol. 1995;252:283-92</Citation><ArticleIdList><ArticleId IdType="pubmed">7476363</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biochem. 1997 May;121(5):842-8</Citation><ArticleIdList><ArticleId IdType="pubmed">9192723</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Cells. 1997 Oct 31;7(5):655-60</Citation><ArticleIdList><ArticleId IdType="pubmed">9387154</ArticleId></ArticleIdList></Reference><Reference><Citation>Comp Biochem Physiol A Physiol. 1997 Nov;118(3):489-93</Citation><ArticleIdList><ArticleId IdType="pubmed">9406431</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 1964 Oct;239:3436-44</Citation><ArticleIdList><ArticleId IdType="pubmed">14245400</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Bot. 2006;57(8):1685-96</Citation><ArticleIdList><ArticleId IdType="pubmed">16720602</ArticleId></ArticleIdList></Reference><Reference><Citation>Mycol Res. 2006 Nov;110(Pt 11):1347-54</Citation><ArticleIdList><ArticleId IdType="pubmed">17070679</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Mol Life Sci. 2007 Jun;64(12):1518-30</Citation><ArticleIdList><ArticleId IdType="pubmed">17415523</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2008 Apr 4;283(14):8868-76</Citation><ArticleIdList><ArticleId IdType="pubmed">18216016</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochim Biophys Acta. 2008 Nov;1780(11):1304-17</Citation><ArticleIdList><ArticleId IdType="pubmed">18621099</ArticleId></ArticleIdList></Reference><Reference><Citation>Extremophiles. 2009 Mar;13(2):273-81</Citation><ArticleIdList><ArticleId IdType="pubmed">19089529</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Biosyst. 2009 May;5(5):519-28</Citation><ArticleIdList><ArticleId IdType="pubmed">19381366</ArticleId></ArticleIdList></Reference><Reference><Citation>Virus Genes. 2009 Dec;39(3):418-26</Citation><ArticleIdList><ArticleId IdType="pubmed">19697117</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Protein Pept Sci. 2010 Dec;11(8):659-68</Citation><ArticleIdList><ArticleId IdType="pubmed">21235502</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Biol Rep. 2012 Sep;39(9):9233-8</Citation><ArticleIdList><ArticleId IdType="pubmed">22714922</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem J. 2012 Sep 15;446(3):333-48</Citation><ArticleIdList><ArticleId IdType="pubmed">22928493</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Genet. 2009;43:335-67</Citation><ArticleIdList><ArticleId IdType="pubmed">19691428</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Wang, Quanfu" sort="Wang, Quanfu" uniqKey="Wang Q" first="Quanfu" last="Wang">Quanfu Wang</name></noRegion><name sortKey="Chen, Qian" sort="Chen, Qian" uniqKey="Chen Q" first="Qian" last="Chen">Qian Chen</name><name sortKey="Han, Xiao" sort="Han, Xiao" uniqKey="Han X" first="Xiao" last="Han">Xiao Han</name><name sortKey="Hou, Yanhua" sort="Hou, Yanhua" uniqKey="Hou Y" first="Yanhua" last="Hou">Yanhua Hou</name><name sortKey="Hu, Zhiguo" sort="Hu, Zhiguo" uniqKey="Hu Z" first="Zhiguo" last="Hu">Zhiguo Hu</name><name sortKey="Li, Yujin" sort="Li, Yujin" uniqKey="Li Y" first="Yujin" last="Li">Yujin Li</name><name sortKey="Liu, Yuanping" sort="Liu, Yuanping" uniqKey="Liu Y" first="Yuanping" last="Liu">Yuanping Liu</name><name sortKey="Shi, Yonglei" sort="Shi, Yonglei" uniqKey="Shi Y" first="Yonglei" last="Shi">Yonglei Shi</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/GlutaredoxinV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000671 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000671 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= GlutaredoxinV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:25110664
|texte= Cloning, expression, purification, and characterization of glutaredoxin from Antarctic sea-ice bacterium Pseudoalteromonas sp. AN178.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:25110664" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a GlutaredoxinV1
| This area was generated with Dilib version V0.6.37. |  |